Question: What is the maximum number of antibodies that can be used when performing Visium with Immunofluorescence?
Answer: 10x has tested and supports up to 4 fluorophores (3 antibodies plus DAPI nuclei stain) at a time with the Immunofluorescence (IF) demonstrated protocol. You can detect up to 6 fluorophores, but the maximum number detected will be limited by imaging system capabilities and microscope filters available.
Products: Visium for fresh-frozen, Visium for FFPE