Question: If I have performed Visium with H&E staining, do I need to repeat tissue optimization with Immunofluorescence (IF) staining?
Answer: Yes, permeabilization times identified using the H&E staining protocol may not be applicable to immunofluorescence staining. We recommend for each tissue type, performing a tissue optimization experiment with each staining method to determine the optimal permeabilization time to be used during the Visium Gene Expression experiment.
Products: Visium for fresh-frozen