Question: Does the transposition reaction damage mRNA or promote nuclei leakage?
Answer: We do not expect that mRNA will be damaged or that high quality nuclei will experience significant leakage during the transposition reaction. We have observed comparable Fraction of Reads in Cells and other quality metrics between nuclei that were isolated using the Single Cell 3’ Gene Expression nuclei isolation Demonstrated Protocol (which does not involve a transposition step) and the Single Cell Multiome ATAC + GEX nuclei isolation Demonstrated Protocol (which involves transposition step).
Please see: Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Data Comparison
Products: Single Cell Multiome ATAC+GEX