Question: How are the ATAC and RNA fragments separated to generate individual Multiome ATAC and GEX libraries?
Answer: All steps including barcoding and pre-amplification of barcoded fragments are performed together. After pre-amplification, a portion of the pre-amplification product is taken into ATAC library constructions. Primers used in the Multiome ATAC library construction will specifically amplify barcoded ATAC products and not barcoded mRNA. Another portion of the pre-amplification product is taken into cDNA amplification and Multiome GEX library construction. Primers used in the Multiome GEX library construction will specifically amplify barcoded mRNA and not barcoded ATAC products. A useful diagram demonstrating the workflow can be found in the stepwise objectives of the User Guide.
Products: Single Cell Multiome ATAC + GEX