Q: Can 5’ v2 libraries be made using single-indices instead of dual-indices?
A: 5' v2 has only been validated on dual-index libraries, for all library types (gene expression, TCR, BCR and Feature Barcode). Single index libraries could be made from 5’ v2 cDNA by mix and matching v1/v1.1 and v2 reagents. However, this is untested and we cannot guarantee the assay performance. In addition, this is not supported by 10x Genomics.
Products: Single Cell Immune Profiling