Q: Can I use my 5’ v1/v1.1 V(D)J primers with 5’ v2 reagent kits?
A: The 5’ v2 TCR/BCR amplification kit primers have been optimized for performance with the 5’ v2 reagent kits. 5’ v1/v1.1 primer sequences may technically be compatible, but we have not tested these with 5’ v2 kits and cannot guarantee assay performance.
Additionally, please note forward primers in our V(D)J primer mixes have been changed to accommodate dual-indexing. Therefore if you wish to use 5’ v1/v1.1 V(D)J primer mixes with 5’ v2 reagents, additional 5’ v1/v1.1 reagents would be needed in order to create single-index libraries.
Products: Single Cell Immune Profiling