Question: How can I easily visualize the Fiducial Frame on the Visium Slide during tissue section placement?
Answer: To easily visualize the Fiducial Frame on the Visium Slide during tissue section placement:
- Trace the fiducial frame(s) on the back of the Visium Slide with a permanent marker. Ensure the sticker at the top of the slide is facing down and not readable.
- After tissue drying and before Deparaffinization, remove the ink with a 100% ethanol-soaked laboratory wipe.
- Ensure the ink is completely removed before Deparaffinization and avoid touching the active surface of the slide when performing the above steps.
Products: Spatial Gene Expression for FFPE