Question: When during the workflow can I use the Visium Spatial Slide Reset Demonstrated Protocol?
Answer: Slides should be reset as soon as a mistake is identified. Visium Tissue Optimization or Gene Expression slides must be reset prior to the tissue permeabilization step. After reverse transcription has occurred, barcodes have been extended into the transcript therefore slides can no longer be reset.
Visium Slides can be reset after any of the following steps:
- Cryosectioning and placing tissue sections
- Methanol fixation
- H&E staining or Immunofluorescence staining
- H&E or Immunofluorescence imaging