Question: How does the Visium Spatial Slide Reset Demonstrated Protocol affect subsequent assay performance?
Answer: Resetting Visium Spatial Gene Expression slides will likely result in a decrease in the number of unique transcripts detected for many tissue types. Similarly, resetting Visium Spatial Tissue Optimization slides may cause a reduction in cDNA footprint fluorescence intensity for certain tissue types; however, this should be uniform across the entire slide and should not affect the determination of optimal permeabilization time.
Additional details regarding assay sensitivity post reset can be found in the Results section of the demonstrated protocol.
Product: Visium for fresh-frozen