Question: Can the same panels be used with 3' and 5' libraries in the Targeted Gene Expression assay?
Answer: Panels used in the Targeted Gene Expression assay consist of baits that hybridize to library fragments corresponding to genes of interest. When designing baits for 10x pre-designed panels and for custom panels (using the 10x Custom Panel Designer), baits are tiled across the entire non-redundant mature transcript space. This includes exons (including UTRs) from all annotated isoforms, and does not include introns.
Given that baits are tiled across the entire mature transcript, the same panel can be used with 3' and 5' Gene Expression libraries.
Example of bait tiling across exons from all annotated isoforms:
Products: Targeted Gene Expression