Question: Can I pool single and dual index libraries for use in the Targeted Gene Expression assay?
Answer: For the Targeted Gene Expression assay, up to eight Whole Transcriptome Analysis (WTA) libraries can be pooled prior to hybridization.
While both single index and dual index WTA libraries are compatible with the Targeted Gene Expression assay, we do not recommend pooling single index and dual index WTA libraries for use in the same targeted assay. Pooling single index and dual index WTA libraries will result in a targeted library that contains a mixture of single index and dual index library fragments. Sequencing mixed pools of single and dual index library fragments are not supported by Illumina.
For further information, please see: Can single index and dual index libraries be pooled for sequencing?
Products: Targeted Gene Expression, Single Cell Gene Expression, Single Cell Immune Profiling