Question: Are dual index plates shared across 10x assays?
Answer: Dual index plates are shared across certain 10x assays. Please use only the dual index plate recommended in the User Guide for each assay and library type. If the incorrect dual index plate is used, library preparation will be unsuccessful.
Dual Index Kit TT Set A (PN-1000215 / PN-3000431):
- 3' v4, 3’ v3.1, 3' HT v3.1, and 3' LT v3.1 Dual Index Single Cell Gene Expression Library
- 5' v3, 5' v2 and 5' HT v2 Dual Index Single Cell Gene Expression Library
- 5' v3, 5' v2 and 5' HT v2 Dual Index V(D)J Library
- 5' v3, 5' v2 and 5' HT v2 CRISPR Screening Library
- Visium Spatial Gene Expression Library
- Single Cell Multiome Gene Expression Library
- Fixed RNA Profiling Cell Surface Protein Library
Dual Index Kit NT Set A (PN-1000242 / PN-3000483):
- 3' v4, 3’ v3.1, 3' HT v3.1, and 3' v3.1 LT Dual Index Cell Surface Protein Library
- 3’ v3.1, 3' HT v3.1, and 3' v3.1 LT Dual Index CRISPR Screening Library
- Visium CytAssist Spatial Protein Expression Library
Dual Index Kit TN Set A (PN-1000250 / PN-3000510):
- 5' v3, 5' v2 and 5' HT v2 Dual Index Cell Surface Protein Library
Dual Index Kit NN Set A (PN-1000243 / PN-3000482):
- 3’ v3.1 and 3' HT v3.1 Dual Index Cell Multiplexing Library
Dual Index Kit TS Set A (PN-1000251/PN-3000511):
- Visium HD Spatial Gene Expression Library
- Visium Spatial Gene Expression for FFPE Library
- Visium CytAssist Spatial Gene Expression Library
- Fixed RNA Profiling Gene Expression Library
- 5' v2 and 5' HT v2 Barcode Enabled Antigen Mapping (BEAM) Library
Also, see: Why are different index plates required for different library types?
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell Multiome ATAC+GEX, Single Cell ATAC, Fixed RNA Profiling, Single Cell Gene Expression Flex, Visium for FFPE, Visium for Fresh Frozen, Visium CytAssist for FFPE, Visium CytAssist Spatial Gene and Protein, Visium HD Spatial Gene Expression.