Question: Are alternative streptavidin beads compatible with the Targeted Gene Expression assay?
Answer: For the Targeted Gene Expression assay, streptavidin beads are used to capture biotinylated baits and their associated library fragments.
The Targeted Gene Expression assay was validated with Dynabeads M-270 Streptavidin (Thermo Fisher Scientific, #65305). These streptavidin beads are recommended for optimal assay performance.
We have performed limited in-house tests on alternative streptavidin beads. Based on these tests, the following streptavidin beads from Thermo Fisher Scientific were found to be compatible with the targeted assay:
- Dynabeads M-280 Streptavidin
- Dynabeads MyOne Streptavidin T1
- Dynabeads MyOne Streptavidin C1
The following streptavidin beads led to reduced assay performance and are not recommended:
- PureProteome Streptavidin Magnetic Beads (Sigma)
- Streptavidin Magnetic Beads (NEB)
Products: Targeted Gene Expression