Question: Is the Targeted Gene Expression assay compatible with other species?
Answer: We have only validated the Single Cell Targeted Gene Expression assay on human and mouse samples. Other species are not currently supported.
Pre-designed panels: 10x pre-designed panels are only available for human genes
Custom panels: The 10x Custom Panel Designer supports human or mouse gene panels
Note: while the 10x Custom Panel Designer can be used to design baits for exogenous (non-human/mouse) sequences, such as GFP reporters, it is not an appropriate tool for designing full panels for other species. No more than 10 exogenous sequences can be entered per custom panel. Furthermore, the bioinformatic filtering steps (to exclude baits mapping to repetitive sequences) were optimized for the human/mouse genome and may not be optimal for other species.
The Spatial Targeted Gene Expression assay has only been validated using human samples with our pre-designed panels. For additional information: Are fully custom panels supported for use in the Spatial Targeted Gene Expression assay?
Products: Targeted Gene Expression