Question: Is the Targeted Gene Expression assay compatible with other species?
Answer: We have only validated the Targeted Gene Expression assay on human samples. Other species are not currently supported.
Pre-designed panels: 10x pre-designed panels are only available for human genes
Custom panels: The 10x Custom Panel Designer only supports human gene panels
Note: while the 10x Custom Panel Designer can be used to design baits for exogenous (non-human) sequences, such as GFP reporters, it is not an appropriate tool for designing full panels for non-human species. No more than 10 exogenous sequences can be entered per custom panel. Furthermore, the bioinformatic filtering steps (to exclude baits mapping to repetitive sequences) were optimized for the human genome and may not be optimal for other species.
Workflow steps: with regards to the workflow itself, one of the reagents used to block non-specific binding is Human Cot DNA. We have not tested whether this is an optimal blocking agent for other species.
We may consider expanding our support to additional species in the future but do not currently have any timelines for if or when this would be available.
Products: Targeted Gene Expression