Question: My lab is shutting down temporarily, how should I store my samples?
Cell suspension and tissue storage for Chromium Single Cell protocols:
If you have not started preparing your samples, the best way to store samples is to freeze them.
Cells can be resuspended in freezing media, as noted in our demonstrated protocols for cell lines and
PBMCs. Long-term storage in liquid nitrogen is recommended. Tissues can be either dissociated and then frozen as single cell suspensions or flash frozen whole (to be later processed as nuclei).
Tissue storage for Visium Spatial protocols:
Tissues that will be processed using the Visium Spatial Gene Expression Solution can be snap-frozen in an isopentane and liquid nitrogen bath and stored in a sealed container at -80°C for long-term
storage. OCT embedded tissue blocks can also be stored in a sealed container at -80°C for long-term
storage. We recommend checking the RNA quality of your tissue block (RIN score) before proceeding with the Visium assay. Protocols for tissue freezing, OCT embedding, storage, and RNA quality assessment can be found in the Visium Tissue Preparation Guide.
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Visium