Question: During GEM-RT Incubation in the Single Cell Assays, can the 85°C (step 2) be extended for more than 5 min?
Answer: It is not recommended to extend step 2 at 85°C beyond 5 min as this can degrade the first-strand cDNA and negatively impact library quality and complexity.
Products: Single Cell Gene Expression, Single Cell Immune Profiling