Question: How can I more easily visualize the fiducial frame during tissue section placement?
Answer: Prior to equilibrating the Visium Gene Expression slide in the cryostat, trace the fiducial frame(s) on the back of the slide with a permanent marker. If the slide was stored at -20°C (verify slide storage temperature on kit label), equilibrate the slide to room temperature first. When marking the slide, ensure the sticker at the top of the slide is facing down and not readable. After performing the 1 min drying step at 37C and before methanol fixation, remove the ink with a 100% ethanol-soaked laboratory wipe. Ensure the ink is completely removed prior to methanol fixation and avoid touching the active side of the slide when performing the above steps.
Products: Visium for fresh-frozen