Question: How much volume is used for library dilutions during the KAPA qPCR setup on the Chromium Connect?
Answer: An input volume of 25ul is suggested for accurate pipetting. The Chromium Connect aspirates two-3 ul aliquots (6 ul total volume) for library dilutions which are then loaded into the qPCR plate. The remaining library volumes can be collected and stored after qPCR setup.
Products: Chromium Connect