Question: Can we run feature barcoding off the remaining Chromium Connect cDNA?
Answer: Although a portion of the Chromium Connect cDNA is saved before library preparation, it is not possible to generate feature barcoding libraries. Feature barcoding requires custom “feature cDNA primers” during the cDNA amplification step and storage of the supernatant during SPRI-bead cleanup. These two steps are not currently part of the automated workflow or reagent kits.
Products: Chromium Connect