Question: What is the sample volume that is loaded onto the Chromium Connect instrument?
Answer: The Chromium Connect requires 10ul of cell suspension per reaction but can handle the same cell numbers as the manual assay of 500-10,000 cells per channel. The recommended cell stock concentration is 700-1200 cells/ul for accurate counting. Refer to the Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits v3.1 User Guide’s (CG000286) Cell Suspension Volume Calculator Table for optimal cell loading guidelines.
Products: Chromium Connect