Question: How much of my cDNA is used for library prep on the Chromium Connect?
Answer: When the mid-run QC is selected, the Chromium Connect will dispense 25ul aliquots of cDNA into a strip tube for the user to perform subsequent QC steps on a Bioanalyzer or Tapestation off-deck. A 15ul fraction (~40%) of the cDNA remains on deck for fragmentation and library preparation.
When the mid-run QC is NOT selected, the cDNA will remain on deck and will not be available for subsequent QC steps. A 15ul aliquot (~40%) of the cDNA will be still be used for fragmentation and library preparation.
The volume is not adjustable if low cDNA yield is observed. However, the SI-PCR cycles can be adjusted during mid-run QC.
Products: Chromium Connect