Question: Can OCT cover the Fiducial Frame or extend outside the capture area?
Answer: OCT can overlap the Fiducial Frame and extend outside the capture area. The OCT will be washed away during the H&E staining steps of the Gene Expression workflow. However, it is important to avoid OCT from one tissue section overlapping into an adjacent capture area. When the excess OCT melts, it can disrupt the morphology of the overlapping tissue section.