Question: Can OCT cover the Fiducial Frame or extend outside the capture area?
Answer: OCT can overlap the Fiducial Frame and extend outside the capture area. The OCT will be washed away during the sample preparation steps Gene Expression workflow. However, it is important to avoid OCT from one tissue section overlapping into an adjacent capture area (for Visium) or tissue section (for Xenium). When the excess OCT melts, it can disrupt the morphology of the overlapping tissue section.
Product: Visium for fresh-frozen, Xenium In Situ Gene Expression