Question: How can I avoid overdrying of my tissue sections?
Answer: Tissue sections can overdry if left exposed in the cryostat for long periods. To prevent overdrying from occurring, the Visium slide can be placed inside a sealed slide mailer or 50ml centrifuge tube pre-chilled and maintained in the cryostat. If working with multiple tissue blocks, we would recommend preparing each tissue block ahead of time for sectioning (eg equilibration and mounting of tissue onto specimen chuck), so that blocks can be interchanged quickly.
Product: Visium for fresh-frozen