Question: Can I section from the same tissue block across multiple days?
Answer: The same tissue block can be taken in and out of storage at -80C for sectioning on multiple days. Ensure to keep the tissue frozen by placing sealed container on dry ice when transporting tissue from the -80C freezer to a pre-cooled cryostat. This allows you to collect sections for RNA quality assessment (RIN score determination) one day and then on another day section tissues from the same tissue block for Tissue Optimization and/or Gene Expression slide placement. Ensure to cap off the tissue block with additional OCT before restoring the tissue block. For additional details on how to store the leftover tissue blocks, please see the Tissue Preparation Guide.