Question: Can I section from the same tissue block across multiple days?
Answer: The same tissue block can be taken in and out of storage at -80C for sectioning on multiple days. Ensure to keep the tissue frozen by placing the sealed container on dry ice when transporting tissue from the -80C freezer to a pre-cooled cryostat. This allows you to collect sections for tissue QC one day and then on another day section tissues from the same tissue block for the Visium/Xenium workflow. Ensure to cap off the tissue block with additional OCT before restoring the tissue block. For additional details on how to store the leftover tissue blocks, please see the relevant documentation listed below:
- Visium Spatial Gene Expression - Tissue Preparation Guide
- Visium CytAssist Spatial Gene Expression for Fresh Frozen - Tissue Preparation Guide
- Xenium In Situ for Fresh Frozen Tissues – Tissue Preparation Guide
Product: Visium for fresh-frozen, Visium CytAssist for FF, Xenium In Situ Gene Expression