Question: Does using a coverslip during imaging affect Visium for fresh-frozen data quality?
Answer: If looking to generate higher resolution images at higher magnifications, the use of a coverslip will lead to improved image quality. Some imaging systems require the use of a coverslip for proper focusing. Visium Gene Expression experiments comparing slides with and without using coverslips during imaging showed no difference in overall clustering and data interpretation.
Product: Visium for fresh-frozen