Question: Can Visium for fresh-frozen libraries be pooled with other 10x libraries?
Answer: Visium for fresh-frozen libraries are dual-indexed and can be pooled with other 10x dual indexed libraries as long as the recommended sequencing configurations are followed. The following libraries can be pooled together for Illumina sequencing:
- Visium Spatial Gene Expression Libraries (Dual Index)
- Chromium Single Cell 3’ v3.1 (Dual Index)
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- Gene Expression Libraries
- Cell Multiplexing Libraries
- Cell Surface Protein Libraries
- CRISPR Screening Libraries
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- Chromium Single Cell 5’ v2 (Dual Index)*
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- Gene Expression Libraries*
- V(D)J Enriched Libraries*
- Cell Surface Protein Libraries*
- CRISPR Screening Libraries*
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- Chromium Single Cell Multiome Gene Expression Libraries (Dual Index)
*If run with 28 cycles for Read 1
Product: Visium for fresh-frozen