Question: What sequencers are Visium libraries compatible with?
Answer: The Visium Solution is compatible with all Illumina sequencers. Because of the sequencing throughput needed (we recommend a minimum of 50k read pairs per spot covered with tissue), NextSeq, HiSeq 2500, HiSeq 4000, and NovaSeq are recommended. iSeq and MiSeq platforms can be used for library QC.
A comparison of sequencing metrics for pooled Visium Spatial Gene Expression libraries across Illumina️ platforms can be found in this Sequencing Metrics & Base Composition of Visium Spatial Gene Expression Libraries Technical Note: https://support.10xgenomics.com/spatial-gene-expression/sequencing/doc/technical-note-sequencing-metrics-and-base-composition-of-visium-spatial-gene-expression-libraries