Question: What sequencers are Visium for fresh-frozen libraries compatible with?
Answer: The Visium Solution has been tested for compatibility with the following Illumina sequencers:
- NextSeq 500/550
- HiSeq 2500 (Rapid Run)
- HiSeq 3000/4000
- NextSeq 1000/2000
- NovaSeq 6000
Because of the sequencing throughput needed (we recommend a minimum of 50k read pairs per spot covered with tissue), NextSeq, HiSeq 2500, HiSeq 4000, and NovaSeq 6000 are recommended. iSeq and MiSeq platforms can be used for library QC.
A comparison of sequencing metrics for pooled Visium Spatial Gene Expression libraries across Illumina️ platforms can be found in this Sequencing Metrics & Base Composition of Visium Spatial Gene Expression Libraries Technical Note.
For more information about sequencing recommendations for Visium for fresh-frozen libraries please see: Sequencing Requirements for Visium Spatial Gene Expression
Product: Visium for fresh-frozen