Question: What does a good Visium Spatial Gene Expression library look like?
Answer: The trace of a good Visium library will appear as a single discrete peak approximating a bell curve 300 to 600bp in size with an average library size of 450bp. You may observe very small low molecular weight peaks (50 to 150bp size range) which are expected. These are low levels of primer carryover remaining after SPRIselect cleanup. This is normal and does not affect sequencing or application performance.
Library traces from mouse brain tissue are provided below. Traces are also provided in the Visium Spatial Gene Expression User Guide.
Bioanalyzer - DNA High Sensitivity Kit
Tapestation - High Sensitivity D5000 Kit
LabChip GX Touch HT - DNA HS Reagent Kit