Question: What sequencing parameters should be used for Visium for fresh-frozen libraries?
Answer: Visium for fresh-frozen libraries should be run paired-end, dual index configuration. We recommend the following sequencing cycling conditions:
Read 1 - 28 cycles
i7 index - 10 cycles
I5 index - 10 cycles
Read 2 - 90 cycles
Final library construct with cycle numbers annotated:
Product: Visium for fresh-frozen