Question: Can I use alternative methods to qPCR, such as Nanodrop or Qubit, for cDNA quantification in the Visium assay?
Answer: qPCR is the only validated method to quantify unamplified cDNA accurately and can't be replaced with alternative methods. The concentration of cDNA is below the level of detection for alternative methods, therefore, qPCR has to be employed to quantitate how many cycles should be performed for cDNA amplification.
Product: Visium for fresh-frozen