Question: Is Visium compatible with cells or tissues expressing GFP/RFP?
Answer: We have not tested in-house whether GFP or RFP expressing tissues are compatible with Visium. This is something we plan to test in the future.
It is unknown whether the GFP signal would be killed by the methanol fixation, but if you were to try this, the fluorescence imaging would most likely need to occur before H&E staining; especially due to the autofluorescence from the H&E.
An alternative solution to using the GFP/RFP signal directly on a Visium slide is to perform the fluorescent imaging on a subsequent section on a plain glass slide and overlay this with the H&E image generated from the Visium slide. Unfortunately, with this method you will only get a general GFP+ cell distribution, as opposed to a direct 1:1 relationship.