Question: What tissue section thickness should I use with the Visium Slides?
Answer: The thickness of sectioned samples for the Visium Slide will depend upon tissue type. Fatty tissues generally require thicker sections. 5 - 35 um sections have been tested in-house, however, 10 um sections are used for most tissue types. Changes in tissue section thickness will require a reassessment of permeabilization time using the Tissue Optimization Kit.