Question: Which thermal cyclers are compatible with the Visium Thermocycler Adaptor?
Answer: In order for the Thermocycler Adaptor to be compatible with a thermal cycler, it requires a 96-well block that fits .2ml PCR tubes or plates (deep-well) and that the lid is able to close once the Visium Thermocycler Adaptor and Slide Cassette(s) are added. A controllable-heated lid is required to run the Visium Tissue Optimization (required only for Visium, not Visium for FFPE) and Gene Expression workflows.
The Thermocycler Adaptor is compatible with the following instruments:
- Bio-Rad C1000*
- Bio-Rad S1000
- Bio-Rad T100
- Eppendorf Mastercycler EP Gradient S
- Eppendorf Mastercycler Pro*
- Eppendorf Vapo Protect
- MJ Research Tetrad 2
- Thermo Fisher Scientific (Applied Biosystems) Veriti*
The Thermocycler Adaptor is not compatible with the following instruments due to the lid not being able to close:
- Applied Biosystems MiniAmp
- Applied Biosystems ProFlex
- Applied Biosystems SimpliAmp
- Thermo Fisher Scientific VeritiPro
If a thermal cycler you are interested in is not on the list above, we have not yet had access to testing it.
*Indicates recommended thermal cyclers that have been validated with the Visium assay
Products: Visium for fresh-frozen, Visium for FFPE