Question: Which thermal cyclers are compatible with the Visium Thermocycler Adaptor?
Answer: In order for the Thermocycler Adapter to be compatible with a thermal cycler, it requires a 96-well block that fits .2ml PCR tubes or plates (deep-well) and that the lid is able to close once the Thermocycler Adapter and slide cassette(s) are added. A heated lid is required to run the Visium Tissue Optimization and Gene Expression workflows.
The Thermocycler Adaptor is compatible with the following instruments:
- Bio-Rad C1000*
- Bio-Rad S1000
- Bio-Rad T100
- Eppendorf Mastercycler EP Gradient S
- Eppendorf Mastercycler Pro*
- Eppendorf Vapo Protect
- MJ Research Tetrad 2
- Thermo Fisher Scientific (Applied Biosystems) Veriti*
The Thermocycler Adaptor is not compatible with the following instruments due to the lid not being able to close:
- Applied Biosystems ProFlex
- Applied Biosystems SimpliAmp
If a thermal cycler you are interested in is not on the list above, we have not yet had access to testing it.
*Recommended thermal cyclers that have been validated with the Visium assay