Question: Do we recommend running replicate tissue sections with Visium?
Answer: For Visium Gene Expression slides, the number of replicates you will need will depend on your research goal and available resources (kits, tissue specimens). The number of replicates will vary depending on the tissue being processed and how variable each consecutive slice is from the last slice- i.e., for a highly heterogeneous tissue block, there may be differences between each 10-micron section sliced, even if they are consecutive.
We typically run at least 2 replicates per tissue type. From our experience, for one tissue type, we see very similar results between two replicates, whereas other tissue types we tested, we see a broader range across four replicates.