Question: What are the imaging system requirements for running Visium?
Answer: The imagining requirements for running Visium are as follows:
a) The three microscope systems listed below were used by 10x Genomics. Any equivalent system with the listed features may be used:
- Nikon Eclipse Ti2 with brightfield and fluorescence capacity (TRITC)
- Molecular Devices ImageXpress Nano Automated Cell Imaging System
b) Microscope features needed:
- Objectives: 4X (Plan APO λ; NA 0.20); 10X (Plan APO λ; NA 0.45); 20X (Plan APO λ; NA 0.75)
- Scanning Stage: Microscope tile scanning functionality is required for imaging tissue sections placed on a Capture Area of a Visium Spatial slide.
- Brightfield Features:
- Color camera (3 x 8 bit, 2424 x 2424 pixel resolution)
- White balancing functionality
- Capture resolution minimum of 2.18 μm/pixel
- Exposure times 2 ms - 10 ms
c) Fluorescence Features (required only for Tissue Optimization workflow):
- Light source (or equivalent) with a wavelength range of 380-680 nm
- Monochrome camera (14 bit, 2424 x 2424 pixel resolution)
- TRITC filter cube (Excitation 542/20, Emission 620/52)
- Capture resolution minimum 2.18 μm/pixel
- Exposure times 100 ms- 2 sec
d) Additional Specifications:
- Computer with sufficient power to handle large images (0.5-5 GB)
- Image stitching software (microscope’s software or equivalent, like Image J)
- Save image in tiff (preferred) or jpeg format
For more details, please refer to our Imaging Guidelines Technical Note
Product: Visium