Question: If I am running a time-course study collecting tissues, what is the best way to section and prepare the Visium Gene Expression slide with tissue?
Answer: There are two options to section and prepare the Visium Gene Expression slide in a time-course study:
- Adding one tissue section to the slide at a time. It is essential to keep the slide cold throughout each sectioning event and during transporting the slide to and from the -80C and cryostat. Condensation formation on the slide is detrimental causing the tissue to disintegrate. Once the tissue section has been placed on the slide, it should be placed in a sealed container such as slide mailer or 50ml conical (separate one per slide) and transported on dry ice to the -80C for storage. Once the first section is placed on the slide it can be stored in a sealed container at -80C for up to 7 days therefore timing of when the sections are to be added needs to be considered.
- Adding all four tissue sections to the slide at one time. Prepare tissue block for each time point by snap freezing with isopentane and liquid nitrogen bath. Place tissue block in pre-chilled sealed container and store at -80C until all time point blocks are collected and properly snap frozen. Then embed the individual blocks in OCT if not done during snap freezing step. Perform sectioning with all the blocks are ready to go and prepare the slide with tissue sections. This slide with the tissue can be stored in a sealed container at -80C for up to 7 days.