Question: What is the Visium Spatial Tissue Optimization Kit (TO) used for?
Answer: The TO kit is used for the first time a customer wants to run a new tissue in their lab. One TO kit can be used to optimize up to 4 tissue samples (4 slides per kit). Each slide has 8 capture areas to test 6 different permeabilization times, a negative permeabilization control, and a positive cDNA synthesis control. Please refer to the Visium Spatial Tissue Optimization user guide for more details and recommended slide layout. The permeabilization time that generates the brightest fluorescent cDNA footprint with the least amount of diffusion is the condition that should be used for the Gene Expression workflow.