Question: Can different tissue types be tested on the Visium Spatial Gene Expression Slide?
Answer: Different tissue types can be tested on the Visium Spatial Gene Expression Slide. Each slide contains four capture areas, therefore, tissue sections from up to four different tissue types can be processed on a single slide. Optimal permeabilization times for each tissue will need to be pre-determined by performing the Tissue Optimization experiment.
If performing H&E staining of your tissue sections, tissue types with different permeabilization times can be processed on a single Visium Gene Expression Slide. If performing immunofluorescence staining, we recommend only processing tissue types with the same permeabilization time on a single Visium slide.