Question: Do we recommend replicates for Visium Gene Expression slides?
Answer: For Visium Gene Expression slides, the number of replicates will vary depending on the tissue being processed, as well as how variable each consecutive slice is from the last slice- i.e. for a highly heterogeneous tissue block, there may be differences between each 10-micron section sliced, even if they are consecutive. We typically run at least 2 replicates per tissue type. In general, the number of replicates will, of course, depend on your research goal and available resources (tissue specimens, kits, sequencing).