Q: How should I store my single-cell suspension?
A: In general, freshly prepared samples will give the best data, followed by cryopreserved and then methanol-fixed samples. Cryopreservation will cause you to lose some cells during the thawing process, but the transcriptomes are comparable (sample-specific). Once you get to methanol fixed, it can be harsh on the cell membranes so you get high degrees of cell lysis and cell loss. We have successfully fixed Jurkat cells, fresh mouse embryonic neuronal tissue, and PBMCs and seen a strong correlation between the transcriptomes, but this will be sample specific. Please note that methanol fixation is not supported with our Single Cell Immune Profiling Solution. It is also not supported with our Feature Barcoding technology.
We don't support other types of fixation, such as formaldehydes--aldehydes disrupt membrane stability, something necessary to retain the "single cell-ness" of the assay. Additionally, the crosslinking and degradation of the nucleic acids may compromise data quality. Even in bulk RNA-Seq, it is common to get degraded RNA from formaldehyde-fixed samples, so these are not recommended for our 10x single cell RNA-seq assays.
Products: Single Cell Gene Expression, Single Cell Immune Profiling