Question: I want to upload my 10x Genomics data to NCBI GEO. What file(s) should I upload?
Answer: For uploading 10x Genomics data to GEO, submitters should upload FASTQ files (both barcode reads and transcript/feature reads) and 10x BAM files, if available. GEO will then submit the raw data files to the Sequence Read Archive (SRA)on your behalf.
If available, SRA encourages the submission of the 10x BAM file (possorted_genome_bam.bam) in addition to the FASTQ files. 10x Genomics offer a tool called bamtofastq for converting 10x BAMs back to FASTQ files that can be used as inputs to re-run the analysis. In addition, SRA pipeline detects 10X BAM files and makes the originally submitted file available from the Download tab of the Run Browser.