Question: Are fresh-frozen tissue samples compatible with Single Cell RNA sequencing?
Answer: If it is not feasible to process fresh tissue, fresh-frozen tissue samples can be used for Single Cell RNA sequencing. Before freezing, the tissue should be dissociated into a single cell suspension. Cells can then be frozen in suitable freezing medium. If the tissue is frozen whole, it is more challenging to isolate viable single cells after thawing.
When freezing cells, we recommend starting with at least 1 million total cells to recover sufficient numbers post-thaw since almost half may be lost in the freeze-thaw process and during the wash and centrifugation steps.
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