Question: Why do I need a different sample index plate (Chromium i7 Sample Index Plate N) to make 5' Feature Barcode libraries?
Answer: For constructing 5' Feature Barcode libraries an additional sample index plate is needed for library construction:
- Chromium i7 Multiplex Kit N, Set A, 96 rxn PN-1000084
The sample index oligos contain a Nextera Read 2 (2N) sequence for priming and amplifying the Feature Barcode molecules during the Sample Index PCR:
- Pooling: The sample indices of Chromium i7 Sample Index Plate (PN-120262) are unique from those of Chromium i7 Sample Index Plate N Set A (PN-1000084). Therefore, respective libraries from the two plates may be pooled.
- Single Cell 3' Gene Expression and 3' Feature Barcode libraries share the same sample index plate, Chromium i7 Multiplex Kit, 96 rxns PN-120262. No additional sample index plate is needed to generate 3' Feature Barcode libraries.
Products: Single Cell Immune Profiling