Question: What is the loading concentration for organisms with small genome sizes?
Answer: For genomes < 1.6 Gb, we recommend loading 0.6 ng total. To do this; first, dilute your sample to 0.5 ng/ul and then following the rest of the User Guide as written. This will result in a final loading concentration of 0.625 ng.
We don't recommend loading less than 0.6 ng total.