Question: Can I use CITE-seq protocols with the Single Cell 5' or 3' workflows?
Answer: CITE-seq is not supported on the 10x Single Cell 3’ or 5’ workflows. However, detecting cell surface protein expression is possible with the Feature Barcoding technology as part of the Single Cell 3' v3 and Single Cell 5' solutions.
For more information, please visit the Single Cell Gene Expression with Feature Barcoding page (Single Cell 3') or the Single Cell Immune Profiling Feature Barcoding page (Single Cell 5').
If interested in performing CITE-seq, we recommend consulting the CITEseq.com website. We haven't validated CITE-seq or REAP-seq in-house, however customers have had success with these applications.
Products: Single Cell 3', VDJ