Question: Where can I order antibody-oligonucleotide conjugates for Feature Barcoding?
Answer: BioLegend offers a selection of antibody-oligonucleotide conjugates that are fully supported for use with 10x Genomics' Single Cell 5' or 3' workflows. The products offered by BioLegend that are compatible are:
- TotalSeq B - fully compatible with Chromium Single Cell Gene Expression Solution with Feature Barcoding Technology (Single Cell 3'v3.1, Single Cell 3' HT v3.1)
- TotalSeq C - fully compatible with Single Cell Immune Profiling Solution with Feature Barcoding Technology (Single Cell 5'v1.1, Single Cell 5' v2, Single Cell 5' HT v2)
Note: TotalSeq A antibodies are designed for CITE-seq and REAP-seq applications and are not supported.
It is also possible to perform custom conjugation of oligos to antibodies of interest for use with the 10x Genomics workflows. See the Demonstrated Protocol 'Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols' for more information: https://support.10xgenomics.com/single-cell-gene-expression/sample-prep/doc/demonstrated-protocol-cell-surface-protein-labeling-for-single-cell-rna-sequencing-protocols.
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Fixed RNA Profiling Gene Expression