Question: What is the recommended amount of PhiX to spike in 10x libraries?
Answer: Our recommendation is to spike-in PhiX with 10x Genomics libraries during the sequencing run. The amount to spike-in depends on the sequencer that is used:
MiSeq, Nextseq and HiSeq2500 platforms: 1%
NovaSeq, HiSeq4000 and HiSeqX: 0.5-1%
Note: For NextSeq with CRISPR Feature Barcoding library run alone, the current recommendation is 20% PhiX
You can always spike in more, but keep in mind that this will reduce the 10x library output.
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Genome/Exome, Single Cell CNV