Question: Can I run samples with low cell viability?
Answer: Samples with low cell viability can have higher background DNA contamination and lead to reduced assay performance.
If background DNA and/or debris in the cell suspension is a concern, we recommend sorting cells prior to nuclei isolation. Do not sort cells after nuclei isolation, as sorting can damage nuclear membranes and lead to more background DNA.
Note: DNase treatment is not recommended because we have observed it negatively impacts the types of cell populations that can be recovered and reduces sample heterogeneity.
Products: Single Cell ATAC